Journal: Cell reports

Article Title: Acetyl-CoA synthesis in the skin is a key determinant of systemic lipid homeostasis

doi: 10.1016/j.celrep.2025.115284

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: InVivo MAb rat monoclonal anti-mouse CD8α , Bio X Cell , Cat#BE0061; RRID: AB_1125541.

Techniques: Western Blot, Staining, Control, Recombinant, Protease Inhibitor, Mass Spectrometry, Bicinchoninic Acid Protein Assay, Acid Assay, Colorimetric Assay, Software, Quantitative Proteomics

Journal: Vaccine

Article Title: SpiN-Tec: A T cell-based recombinant vaccine that is safe, immunogenic, and shows high efficacy in experimental models challenged with SARS-CoV-2 variants of concern.

doi: 10.1016/j.vaccine.2024.126394

Figure Lengend Snippet: Fig. 2. SpiN-Tec immunogenicity and protection against Wuhan isolate in mice model. (a) Total anti-RBD, anti-N, and anti-SpiN IgG titers in mice that received CTVad + Vehicle (black circles), SpiN-Tec 10 μg (light blue circles) or SpiN-Tec 20 μg (dark blue circles) after first immunization (prime) or two doses (prime/boost) [n = 4 mice/group]. (b) Anti-RBD, anti-N, and anti-SpiN IgG1 titers in mice that received CTVad + Vehicle (black circles), SpiN-Tec 10 μg (light blue circles) or SpiN- Tec 20 μg (dark blue circles) after first immunization (prime) or two doses (prime/boost) [n = 4 mice/group]. (c) Anti-RBD, anti-N, and anti-SpiN IgG2c titers in mice that received CTVad + Vehicle (black circles), SpiN-Tec 10 μg (light blue circles) or SpiN-Tec 20 μg (dark blue circles) after first immunization (prime) or two doses (prime/boost) [n = 4 mice/group]. IFN-γ, TNF, IL-6, IL-17 (d) and IL-2, IL-4, and IL-10 (e) produced by splenocytes from CTVad + Vehicle (black circles) or SpiN-Tec 10 μg (blue circles) mice after 72 h of incubation with medium (negative control) and restimulation with recombinant RBD, N or SpiN protein [n = 7 mice/group]. (f) Number of CD4+ and CD8+ T cells producing IFN-γ from splenocytes of mice that received CTVad + Vehicle (black circles) or were immunized with SpiN-Tec 10 μg (blue circles), after restimulation in vitro with SpiN protein for 24 h [n = 7mice/group]. Body weight (g) and survival (h) of K18-hACE2 mice that received CTVad + Vehicle (black lines) or SpiN-Tec 10 μg (blue lines) and K18-hACE2/B-KO that received CTVad + Vehicle (gray lines) or SpiN-Tec 10 μg (light blue dotted lines) and were challenged with the Wuhan isolate of SARS-CoV-2 [K18-hACE2: n = 4 mice/group; K18-hACE2/B-KO: 6–10 mice/group] [* K18-hACE2: CTVad + Vehicle vs SpiN-Tec]. The statistical analysis of cytokines production and IFN-γ producing T cells was carried out using Mann-Whitney U test, lines represent median with an interquartile range. The statistical analysis of body weight curves was performed by area under the curve followed by the Kruskal-Wallis test followed by the post-hoc Dunn’s test. The statistical analysis of survival was performed using the log-rank test. *p < 0.05; **p < 0.01; ***p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Mice were treated on days − 3, − 2 and − 1 before the infection with i.p. injection of 0.5 mg/mouse of rat anti-mouse CD4a mAb (clone GK1.5; Cat. # BE0003–1; BioXCell) and 0.5 mg/mouse of rat anti-mouse CD8a mAb (clone 2.43; Cat. # BE0061; BioXCell) or 0.2 mg/mouse of rat anti-KLH IgG (clone LTF-2; Cat. # BP0090; BioXCell).

Techniques: Immunopeptidomics, Produced, Incubation, Negative Control, Recombinant, In Vitro, MANN-WHITNEY

Journal: Cell metabolism

Article Title: Tumor-associated macrophages are shaped by intratumoral high potassium via Kir2.1.

doi: 10.1016/j.cmet.2022.08.016

Figure Lengend Snippet: Figure 3. Kir2.1 deficiency remodels the intratumor immune landscape (A–D) Schematic of the scRNA-seq experiment design (A). Proportions of different cell types (B). UMAP plot clustering (C). UMAP plot showing Ccl2hi TAM cluster and Kcnj2 expression (D). (E–J) UMAP of VISION-based scoring with indicated GO terms (E and I). Schematic of VISION-based analysis (F). Scatterplots showing the average signature score of each cluster in Kcnj2f/f (WT) and Kcnj2CKO (KO) tumors (G and J). Dot plot of genes from RESPONSE TO INTERFERON BETA gene set and violin plot showing the signature score of the Ccl2hi TAM cluster (H). (K) ECDF plot and violin plot of VISION enrichment score for indicated gene sets. (L and M) Dot plots of selected genes from effector T cell signatures (L). ECDF plot and violin plot of VISION enrichment score of effector CD8+ T cluster (M). (N) Comparison of the significant ligand-receptor involved in TNF-a and IFNg signaling sending from effector CD8+ T cells to other immune cells. (O) Transcripts from Tcf7hi CD8+ T cluster assessed for the enrichment of signatures of memory-precursor T cells. Wilcoxon rank-sum test (H, K, M, and O); one-sided permutation test (N). See also Figure S4.

Article Snippet: For the in vivoCD8 T cell depletion study, mice were intraperitoneally injected with 150 mg rat anti-CD8a mAb (BioXCell, BE0061) or rat IgG2a isotype control (BioXCell, BE0089) 3 days prior to tumor inoculation (day 0), and at days 3, and 6 after s.c tumor injection.

Techniques: Expressing, Comparison

Journal: Vaccine

Article Title: A listeriolysin O Subunit Vaccine is Protective Against Listeria monocytogenes

doi: 10.1016/j.vaccine.2020.06.049

Figure Lengend Snippet: Mice were immunized at weekly intervals for 3 consecutive weeks by intraperitoneal injection of PBS (negative control), or LLOT (20 μg) plus CT (1 μg). Mice received 300 μg of CD4 plus CD8 depleting antibodies or 300 μg of corresponding isotype controls on day 26 via intraperitoneal injection (600 μg total). On day 28, mice were intravenously inoculated with 2 × 104 L. monocytogenes. Mice were given a second 100 μg dose of depleting antibodies or isotype control antibodies 24 h post-infection (200 μg total). Mice were sacrificed after 72 h of infection to collect organs and enumerate bacterial colony forming units (CFUs) in the liver (A) and spleen (B). Data are from 1 experiment with 4 males and 2 females per experimental condition. Results are expressed as CFUs per organ and medians are presented. Statistical significance was calculated using a two-sided Mann-Whitney test, N.S. = Not statistically significant, * P < 0.05 and ** P < 0.01.

Article Snippet: For T cell depletions, mice were treated with 300 μg of rat anti-mouse CD4 monoclonal antibody (mAb) (clone GK1.5, BioXcell) plus 300 μg rat anti-mouse CD8α mAb (clone 53–6.7, BioXcell) by intraperitoneal injection 48 h before infection.

Techniques: Injection, Negative Control, Control, Infection, MANN-WHITNEY